high speed cameras  (Vision Research Inc)

Journal: Ultrasonics Sonochemistry

Article Title: Tuning endothelial barrier permeability with ultrasound: a pulse-length-dependent interplay between bubble dynamics and cellular bioeffects

doi: 10.1016/j.ultsonch.2026.107851

Figure Lengend Snippet: Experimental setup and design . (A) The morphology, (B) Size distribution and (C) Stability of home-made microbubbles with a lipid shell and perfluoropropane core, d90: the size below which 90% of the particles fall within. (D) Characterization of the acoustic field produced by the 1.125 MHz ring ultrasound transducer in the X-Y focal plan. The acoustic pressure is shown in dB relative to the peak value. (E) Experimental setup for ultrasound stimulation, imaging of bubble dynamics and cellular bioeffects inside three parallel microchannels on an inverted microscope. The insets show the enlarged side view and bottom view of the alignment of the ring ultrasound transducer with the microchannels outlined by the red dashed box. (F) Schematic of the ultrasound waveforms used in the experiments: long pulse and rapid short pulse mode. (G) Recording sequences for high-speed imaging of the bubble dynamics and synchronization with ultrasound exposure in short and long pulse mode. (H) Concurrent fluorescent imaging of membrane poration (PI) and calcium signaling (Fluo-4), and synchronization with 10 s ultrasound exposure in short and long pulse mode with a 20 s baseline recording.

Article Snippet: Bubble dynamics was recorded with a 63 × objective (LD PN 63×/0.75 Corr) using a high-speed camera (Nova S12, Photron) that was synchronized to the ultrasound pulses ( ).

Techniques: Produced, Imaging, Inverted Microscopy, Membrane

Journal: Ultrasonics Sonochemistry

Article Title: Tuning endothelial barrier permeability with ultrasound: a pulse-length-dependent interplay between bubble dynamics and cellular bioeffects

doi: 10.1016/j.ultsonch.2026.107851

Figure Lengend Snippet: The dynamic behavior of microbubbles within microchannels under short pulse ultrasound exposure with a flow rate of 75 µL/min. (A) High speed recordings of bubble population dynamics and spatial distribution under 10 consecutive ultrasound pulse exposure (pulse length 40 µs, pulse interval 1 ms) at 0.25 MPa and 0.5 MPa acoustic pressure. (B-C) The enlarged view of the blue and green dashed box in (A) showing the displacement, volume change and coalescence of individual microbubbles immediately before and after the application of each ultrasound pulse in a 10-pulse train at 0.25 MPa and 0.5 MPa acoustic pressure, respectively. The ultrasound pulse arrives at t = 0 and repeats every 1000 μs. (D) The cumulative displacement of the two microbubbles labeled with red arrows in (B) (0.25 MPa) and (C) (0.5 MPa) as a function of time. The exemplary bubble at 0.25 MPa (as shown in B) stopped displacement on the 3rd pulse after it grew to a certain size and then kept oscillating at the same location.

Article Snippet: Bubble dynamics was recorded with a 63 × objective (LD PN 63×/0.75 Corr) using a high-speed camera (Nova S12, Photron) that was synchronized to the ultrasound pulses ( ).

Techniques: Labeling

Journal: Ultrasonics Sonochemistry

Article Title: Tuning endothelial barrier permeability with ultrasound: a pulse-length-dependent interplay between bubble dynamics and cellular bioeffects

doi: 10.1016/j.ultsonch.2026.107851

Figure Lengend Snippet: The dynamic behavior of microbubbles within microchannels under long pulse ultrasound exposure at a flow rate of 75 µL/min. (A) High speed recordings of the bubble dynamics under one long pulse exposure (pulse length 0 – 9.090 ms) at 0.25 MPa and 0.5 MPa acoustic pressure. Ultrasound begins at t = 0. (B) The enlarged view of the bubble dynamics in the green dashed box in (A) showing the displacement and coalescence of microbubbles and (C) a typical example showing the gradual clustering of microbubbles and (D) surface oscillation of an individual microbubble during the long pulse ultrasound exposure at 0.25 MPa. (E) The enlarged view of the bubble dynamics in the blue dashed box in (A) showing the displacement, oscillation and coalescence of microbubbles during the long pulse ultrasound exposure at higher acoustic pressure of 0.50 MPa. (F) Enlarged view of the evolution of the microbubble outlined by brown color (E) at different timings and stages. S1: the microbubble was generated by coalescence of bubble clusters. S2: notable and stable oscillation of the microbubble in the acoustic field. S3: coalescence of the microbubble with another nearby bubble. S4: no discernable oscillation and displacement at this stage even though ultrasound is still on. Please note that S3 has a smaller magnification. (G) Time evolution of the measured bubble diameter corresponding to stage S1-S4 shown in panel F. The inset shows the enlarged view of bubble diameter oscillation in S2.

Article Snippet: Bubble dynamics was recorded with a 63 × objective (LD PN 63×/0.75 Corr) using a high-speed camera (Nova S12, Photron) that was synchronized to the ultrasound pulses ( ).

Techniques: Generated